Catalytic Antibodies, Hapten Designing and Their Applications. Pp. 1-8.
Parveen Salahuddin
[Abstract]
Papers
Determination of the pKa of a Glutamic Residue in a Protein by Difference
Titration Capillary Electrophoresis. Pp. 9-14.
Gonzalo de Prat-Gay
[Abstract]
Optimization of Cultivation of Time for Recombinant Protein Expression from
Yeast Using alphaF Signal Sequence. Pp. 15-18.
Yoshio Hashimoto, Noriko Koyabu and Taiji Imoto
[Abstract]
Effect of Replacement of the Conserved Tyr75 on the Catalytic Properties of
Procine Pepsin A. Pp. 19-26.
Takuji Tanaka, Karen S.L. Teo, Kyra M. Lamb, Linda J. Harris and Rickey Y. Yada
[Abstract]
A Comparative Study of the Specificity of Proinsulin Hydrolysis by Duodenase,
Trypsin and Plasmin. Pp. 27-32.
Olga Mirgorodskaya, Galina Kazanina, Peter Roepstorff, Ekaterina Mirgorodskaya,
Tatyana Zamolodchikova, Tatyana Vorotyntseva, Anatolii Miroshnikov and
Sergey Alexandrov
[Abstract]
Identifying Antigenic Region of Hepatitis B Surface Antigen by Patient's Serum with Random Peptide Library. Pp. 33-40.
Zhi-Jian Yao, Lay-Hain Ong, Lily Chan and Maxey CM. Chung
[Abstract]
Molecular Mechanics Studies on Conformationally Restricted beta-Analine Analogs. Pp. 41-48.
Shashidhar N. Rao and V.N. Balaji
[Abstract]
The Hexapeptide in the alpha Domain of Humanmetallothonein-III may Protrude from the Surface. Pp. 49-52.
Daqing Wu, Joing Shen and Binggen Ru
[Abstract]
Influence of Long-Range Interactions of alpha-Helix Formation. Pp. 53-56.
Cao Aoneng, Sha Yinlin, Kin Dawei, Lai Luhua and Tang Youqi
[Abstract]
[Back to top] Catalytic Antibodies, Hapten Designing and Their Applications. Parveen Salahuddin.
Catalytic antibodies offer a new approach for the catalysis of novel reactions that was heretofore not possible by enzymes. Through careful hapten designing approach the catalytic efficiency of abzymes can excede that of analogous enzymes. They are important analytical tools for destroying specific pathogens or tumor cells [1-2]. Recently they have been used to explore the active site residues of enzyme and in measuring the raised level of enzyme in disease [3-4] using antiidiotypic approach.
[Back to top] Determination of the pKa of a Glutamic Residue in a Protein by Difference Titration Capillary Electrophoresis. Gonzalo de Prat-Gay.
The pKa of residue Glu-45 of chymotrypsin inhibitor-2 has been determined by combining site-directed mutagenesis and capillary electrophoresis. A pKa value of 3.9 is obtained from a plot of the differential mobility of wild-type and mutant chymotrypsin inhibitor-2 versus pH. This value is in excellent agreement with a pKa of 3.7 obtained by NMR titration. The method described here is more accessible than NMR, requires small amounts of sample, and proteins of higher molecular weight, i.e., enzymes, may potentially be analyzed.
[Back to top] Optimization of Cultivation of Time for Recombinant Protein Expression from Yeast Using alphaF Signal Sequence. Yoshio Hashimoto, Noriko Koyabu and Taiji Imoto.
The yeast alpha factor (alphaF) signal sequence is a good signal for the secretion of foreign proteins by yeast. The lysozyme gene was linked after alphaF signal sequence and was introduced into yeast expression vector. The misprocessed subproducts from the transformed yeast increased as cultivation time lengthened. When the cultivation time is shortened, the correctly processed product can be obtained effectively and purely by use of alphaF signal.
[Back to top] Effect of Replacement of the Conserved Tyr75 on the Catalytic Properties of Procine Pepsin A. Takuji Tanaka, Karen S.L. Teo, Kyra M. Lamb, Linda J. Harris and Rickey Y. Yada.
Replacement of a conserved Tyr 75 on the flap loop of pepsin resulted in a change of the catalytic rate. pH dependency an CD analyses indicated, howerer, that the effect was not global. Molecular modelling calculation suggested that the replacements allowed the loop to assume another position thereby changing the catalytic efficiency.
[Back to top] A Comparative Study of the Specificity of Proinsulin Hydrolysis by Duodenase, Trypsin and Plasmin. Olga Mirgorodskaya, Galina Kazanina, Peter Roepstorff, Ekaterina Mirgorodskaya, Tatyana Zamolodchikova, Tatyana Vorotyntseva, Anatolii Miroshnikov and
Sergey Alexandrov.
The comparative specificity of trypsin, plasmin and duodenase in respect to proinsulin hydrolysis was evaluated The sites and the kinetic parameters of the duodenase- and plasmin-catalyzed hydrolysis were shown to differ from those of trypsinolysis. Duodenase and plasmin in contrast to trypsin only inactivate proinsulin, but cannot produce the intermediates leading to the formation of active insulin, because for these enzymes the breaks by the Lys instead of Arg-residues are by far more typical.
[Back to top] Identifying Antigenic Region of Hepatitis B Surface Antigen by Patient's Serum with Random Peptide Library. Zhi-Jian Yao, Lay-Hain Ong, Lily Chan and Maxey CM. Chung.
By screening with random peptide library against human anit-HBsAg antibody, a dominant antibody-binding region was noted. Through peptide synthesis and binding tests, a peptide, corresponding to residues 107-126 and coinciding with a predicted loop region, has been proved to exhibit strong binding capability and the binding could be competitively inhibited by HBsAg. Subsequently, the contributions of each amino acid, sited on this segment were further investigated by alanine scanning.
[Back to top] Molecular Mechanics Studies on Conformationally Restricted beta-Analine Analogs. Shashidhar N. Rao and V.N. Balaji.
We present molecular mechanics conformational energy calculations on the model peptides with 2-aminocyclopropane 1-carboxylic acid (1) and 2-aminocyclobutane-1-carboxylic acid (2). The essential results of the calculations have been presented as plots drawn as a function of the backbone torsion angles phi and psi. The low energy structures of these model compounds provide useful insights into the design of peptidomimetics with turn characteristics.
[Back to top] The Hexapeptide in the alpha Domain of Humanmetallothonein-III may Protrude from the Surface. Daqing Wu, Joing Shen and Binggen Ru.
A main difference between human metallothionein-III and other MT's is that there are additional six amino acid residues in the alpha domain of MT-III. Here, we give some theoretic and experimental evidences for the hypothesis that the insertion of EAAEAE may protrude from the tail of the alpha domain.
[Back to top] Influence of Long-Range Interactions of alpha-Helix Formation. Cao Aoneng, Sha Yinlin, Kin Dawei, Lai Luhua and Tang Youqi.
The alpha-helix fragment in Eglin C was designed to interact with a beta-hairin to examin the influences of long-range interaction on the conformation of this fragment. CD spectra show that one of these designed peptides froms an alpha-beta-beta structure.