Effect of Introducing p-Fluorophenylalanine and Multiple Tryptophan
Residues in a 13-Residue Antibacterial Peptide. Pp. 67-72.
Erugurala Bikshapathy, Narasimhaiah Sitaram and Ramakrishan Nagaraj
[Abstract]
Unusual Combination of the Distorted Structure and Frozen Internal Mobility
in Inactivated Actin Molecule. Pp. 73-78.
Konstantin K. Turoverov, Irina M. Kuznetsova, Sofia Yu. Khaitlina
and Vladimir Uversky.
[Abstract]
Unfolding and Disassociation of the Flg/s28
Complex are Thermodynamically Coupled. Pp. 79-86.
G. W Daughdrill
[Abstract]
Inverse Peptide Synthesis in Solution Using Free Amino Acids as Amino
Components. Pp. 87-90.
Mitin Yu. V and Ryadnov M. G.
[Abstract]
Molecular Dynamics Simulation of the bZIP Domain of the c-JUN Homodimer.
Pp. 91-98.
Yong-Hoon Choi, Chul-Hak Yang, Hyun-Won Kim and Seunho Jung
[Abstract]
Shuffling Algorithm for Protein Design. Pp. 99-104.
Szymon Kaczanowski, Andrzej M. Kierzek and Piotr Zielenkiewicz
[Abstract]
Effect of Acetaldehyde on Aggregation of Neuronal Tau. Pp. 105-110.
Jian-Ying Luo and Rong-Qiao He
[Abstract]
Crystallisation Report
Improved Crystals of Bovine Neurophysin II Complexed with Vasopressin.
Pp. 111-114.
Bing Hu, John P. Rose, M. Gary Newton, Esther Breslow, Changshen
Zheng and Bi-Cheng Wang
[Abstract]
[Back to top] Effect of
Introducing p-Fluorophenylalanine and Multiple Tryptophan Residues in a
13-Residue Antibacterial Peptide. Erugurala Bikshapathy, Narasimhaiah Sitaram
and Ramakrishan Nagaraj.
The effect of introducing tryptophan residues and p-fluorophenylalanine
on the biological activities of the 13-residue antibacterial peptide PKLLTKFLKSWIG
was investigated. Introduction of one and two Trp residues and replacement
of Try and Phe by p-fluorophenylalanine resulted in enhanced antimicrobial
activity as compared to the parent peptide.
[Back to top] Unusual
Combination of the Distorted Structure and Frozen Internal Mobility in
Inactivated Actin Molecule. Konstantin K. Turoverov, Irina M. Kuznetsova,
Sofia Yu. Khaitlina and Vladimir N. Uversky.
The structural properties of inactivated actin were studied by means
of circular dichroism and fluorescence techniques. We have shown that inactivated
actin, being denatured, is characterized by a relatively rigid microenvironment
of aromatic amino acid residues and exhibits a considerable limitation
in the internal mobility of tryptophans. This can be due to the specific
self-association of partially folded protein molecules.
[Back to top] Unfolding
and Disassociation of the Flg/s28
Complex are Thermodynamically Coupled. G. W Daughdrill.
FlgM and s28 regulate the transcription
of flagella and chemotaxis genes in E. coli and S. typhimurium.
In an attempt to deterrnine the strength of the interaction between FlgM
and s28 thermal unfolding studies
were perfonned using circular dichroism spectroscopy (CD). A single, cooperative,
melting transition was observed for the FlgM/s28
complex suggesting that unfolding and disassociation of the complex occur
simultaneously.
[Back to top] Inverse
Peptide Synthesis in Solution Using Free Amino Acids as Amino Components.
Mitin Yu. V and Ryadnov M. G.
We have realized inverse peptide synthesis in order to exclude deprotection
reaction at every synthesis stage. This became possible using high solubility
of free amino acids in dimethylformamide containing Ba(ClO4)2,
Ca(ClO4)2 or Ca(NO3)2. The
developed method was tested on synthesis of [Leu3]-enkephaline,
pentapeptide (1-5) of cholecystokinin, pentapeptide (1-5) of indolicidin,
hexapeptide of phekellistatin 7 and caeridin 1.1.
[Back to top] Molecular
Dynamics Simulation of the bZIP Domain of the c-JUN Homodimer. Yong-Hoon
Choi, Chul-Hak Yang, Hyun-Won Kim and Seunho Jung.
Molecular dynamics simulation of the bZIP domain of the c-Jun homodimer
has been performed. The conformational analyses of leucine zipper domain
showed high consistency to the solution structure obtained from NMR spectroscopy
[2]. The basic domain of homodimer exhibited a Gaussian distributed inter-domain
wagging motion. Constant wagging motion of the basic domain was proposed
to "grope" for DNA major grooves to recognize a specific sequence.
[Back to top] Shuffling
Alghorithm for Protein Design. Szymon Kaczanowski, Andrzej M. Kierzek and
Piotr Zielenkiewicz.
A new algorithm producing a large number of divergent sequences which
can adopt a defined structure is proposed. The algorithm can be applied
to proteins of any size. This is achieved by constraining the amino acid
composition to the native one and minimising the pseudoenergy function
by "shuffling" the sequence. The algorithm is tested by sequence design
for five different protein structures. Modelling by homology with the use
of resultant sequences produces "proper" protein structures as judged using
several different methods.
[Back to top] Effect of
Acetaldehyde on Aggregation of Neuronal Tau. Jian-Ying Luo and Rong-Qiao
He.
Acetaldehyde at a low concentration (0.25%) induces recombinant tau
polymerization distinguishably without using chemical cross-linker disuccinmidyl
suberate (DSS). The aggregation of the hyperphosphorylated tau occurs in
the solution of acetaldehyde at 0.05%. It appears that formation of neurofibrillary
tangles in alcoholics may be correlated to acetaldehyde besides alcohol,
and the abnormal tau phosphorylation may accelerate acetaldehyde-induced
tau polymerization.
[Back to top] Improved
Crystals of Bovine Neurophysin II Complexed with Vasopressin. Bing Hu,
John P. Rose, M. Gary Newton, Esther Breslow, Changshen Zheng and Bi-Cheng
Wang.
A modified bovine neurophysin II in which the first six residues have
been removed by digestion has been crystallized with the hormone vasopressin.
The crystals belong to space group P6122 (or P6322)
with unit cell constants of a = b = 50.9Å, c = 105.8Å. Crystals
of the vasopressin complex have one subunit of the complex per asymmetric
unit, which corresponds to a solvent content of approximately 40%. The
crystals diffract to beyond 2.3Å resolution, which is substantially
better than the 4 Å diffraction reported for the full-length neurophysin-vasopressin
complex reported previously.